Microbiology


https://sites.google.com/a/gauhati.ac.in/research/micro/#nb03092018 Glutamate metabolism in mycobacteria

Glutamate metabolism in mycobacteria

Nabanita Bhattacharyya and her fellow researchers show a way to control the glutamate metabolism in tuberculosis (TB) mycobacteria, which may prove to be a possible mechanism in controlling drug-resistant TB. This work is published in the journal mBio.




Authors
Nabanita Bhattacharyya, Irene Nailain Nkumama1, Zaccheus Newland-Smith1, Li-Ying Lin, Wen Yin
1
, Rebecca E Cullen
1
, Jack S Griffiths
1
, Alexander R Jarvis
1
, Michael J Price
1
, Pei Ying Chong
1
, Russell Wallis
1
, and Helen M O’Hare
1
1 from other institutions

Abstract
Tuberculosis (TB) is responsible for the death of almost 5,000 people every day, and the increasing prevalence of multidrug-resistant TB in the present world has become a matter of utmost concern. The mechanism by which the causal organism of TB Mycobacterium tuberculosis senses and responds to environmental stimuli have proven to be attractive targets for drug development. Recently, the TB research group of Dr. Helen M. O’Hare, in the Department of Infection Immunity and Inflammation (University of Leicester) showed that nutrients stimulate phosphorylation of protein GarA, which is a substrate of the protein kinase G (PknG) in Mycobacterium smegmatis and Mycobacterium tuberculosis and that the phosphorylation status of GarA regulates the central metabolism. In this investigation, we have presented the mechanism by which nutrients activate PknG. Two unknown genes are identified as co-conserved and co-expressed with PknG, whose products are a putative lipoprotein, GlnH, and putative transmembrane protein, GlnX. By using a genetic approach, the authors show that the membrane protein GlnX is functionally linked to PknG. They also propose that the amino acid concentration in the periplasm is sensed by GlnH and that protein-protein interaction allows transmission of this information across the membrane via GlnX to activate PknG.

Journal Reference
https://sites.google.com/a/gauhati.ac.in/research/micro/#top